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FLEX2 On-Line Autosampler: Fully Automated Sampling and Analysis of Key Cell Culture Parameters

Duration: 1 hour, 17 minutes

The BioProfile FLEX2 On-Line Autosampler (OLS) is a modular system that connects as many as 10 bioreactors to a FLEX2 analyzer for automated sampling and analysis of key cell culture analytes including pH, gases, metabolites, osmometry, cell density, and cell viability. The OLS’ small sample volume and fast analysis time provide automated sampling and analysis of 10 bioreactors in less than 1.5 hours. Setup time for a full, 10-bioreactor system requires less than 20 minutes. With proven data comparability to manual sampling methods, the FLEX2 OLS can be connected to OPC-compliant control systems for automated sampling, analysis, and feedback control. Explore this webinar to get a detailed picture of how the FLEX2 OLS can improve your cell culture analysis operation.

Speakers

Matt McRae
Product Line Manager, Biotechnology Products
Nova Biomedical

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Data rich cell line screening: Leveraging ambr® 15 cell culture and BioProfile® FLEX2 integration

Rahul Pradhan, Senior Scientist, Kymab, will present a case study on a manufacturing cell line screening campaign for a Kymab monoclonal antibody fusion protein. The data set obtained by the integration of the BioProfile FLEX2 with ambr 15 helped final cell line selection and process scale-up.

A major challenge of running microscale bioreactor systems like the ambr 15 is the ability to generate a full profile of offline cell culture and metabolite data such as cell counts, pH, pCO2, glucose, lactate and ammonia covering the entire duration of the run due to limited culture volume available for sampling. The combination of the FLEX2 and the ambr 15 solves this problem.The webinar will include examples of how to leverage the feedback loop between the FLEX2 and ambr 15 to allow the generation of a high-quality data set from multiple cell lines, including a full set of cell culture and metabolic profiles, something previously only possible with larger scale bioreactor systems. Additionally, complex process controls can now be implemented in the ambr 15 microbioreactor system by utilising the interconnectivity with the FLEX2 with minimal operator input.

The availability of this rich data set during cell line screening campaigns aids the selection of cell lines more suited to larger scale manufacturing and streamlines development activities.”

Presenters: Rahul Pradhan Senior Scientist – Cell Culture Development Kymab


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